Molecular biology – Gene GPR143. Molecular biology – Gene GPR143. Your paper should include as much of the following information as possible. You should provide adequate background material to acquaint a scientific reader of the nature/function of your gene, mRNA & protein, any important biological features and its implication in the disease state(s).
Each section should have a thorough written discussion of the properties/biology/function etc. You should not just dump data/tables etc. from NCBI, the information needs to be put in a biological context. You need to explain things in your own words.
All figures/tables/cartoons etc. should have a title and a caption explaining the important points you’re making with that figure.
For one figure, create a representation of a protein related to your gene (i.e. related structure) which illustrates some functional aspect of your protein (i.e. a binding site, a protein interface, catalytic residues etc.) using Jmol, then save an image of your creation (for use in the paper).
***My gene is GPR143, and the related protein (selected through PSIPRED) is this:
The figure is to be designed using JMOL (where you can select what to view in the protein, how to view it, colorize, etc)
Don’t start with “The purpose of this paper …”, do not give a detailed rationale or list of methods performed in your abstract.
Proper punctuation, spelling, appropriate word choice and a high standard of grammar are expected. Papers lacking in these attributes will be downgraded.
10 Points Intro
Abstract (A very brief description of your gene/protein and the main
findings of your research)
10 Points Gene Information
Gene Expression (where & when is it expressed)
Gene Regulation (how is expression controlled, feedback
10 Points RNA Information
Alternatively Spliced forms (if any, why are they important?)
RNA regulation (i.e. start sites, half-life, siRNAs etc.)
10 Points Protein Information
Protein sequence (& Protein ID)
Structural/Functional domains within polypeptide
Form of protein (i.e. monomer, homodimer, heterodimer etc.)
Functional Motifs (Prosite https://www.expasy.ch)
Protein half-life (degradation)
Structures of proteins that may be related
Other proteins or small molecules that may interact with your protein
Functional Information (pathways, networks, protein complexes)
15 Points Disease
Disease state(s) for which the gene is implicated
Discuss the nature of the disease (on a molecular basis what is going on), in terms of what you have learned about the control and function of the gene, the mRNA and the protein
What mutations associated with the disease?
Can disease be correlated with structural changes in your protein?
This section should integrate the information you have researched in terms of the gene/rna/protein, and how this information is implicated in the disease state.
5 Points Conclusions & References (at least 10 Refs. Including journals, books, websites etc.) State main findings and tie results together. What direction is current research taking? What future treatments may be effective?
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Molecular biology – Gene GPR143